A 3-step Mechanical Digestion Method to Harvest Adipose-derived Stromal Vascular Fraction
نویسندگان
چکیده
منابع مشابه
Adipose derived stromal vascular fraction improves early tendon healing: an experimental study in rabbits
Tendon never restores the complete biological and mechanical properties after healing. Bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. Stromal vascular fraction (SVF), derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differ...
متن کاملAbstract: Mechanical Isolation of Adipose-Derived Stromal Vascular Fraction: Is It Becoming a Growing Trend?
MaterialS and MethodS: Adults undergoing liposuction on bilateral body areas were included (n=12). Under sedation, liposuction on one side was conducted with standard tumescent (1L of LR with 30ml of 1% lidocaine and 1 mcg/ml epinephrine). Tumescent without lidocaine was infiltrated on the contralateral side. Five milliliter lipoaspirate samples were processed for isolation of the stromal vascu...
متن کاملEffects of Uncultured Adipose Derived Stromal Vascular Fraction on Tendon Healing in Rabbits: A Histological and Immunohistochemical Study
Objective- To evaluate the potential effects of uncultured adipose derived stromal vascular fraction on tendon healing. Design- Prospective descriptive study. Animals- Twenty five adult male New Zealand white rabbits, weighing 2.5-3.0 kg were used. Five rabbits were used as donors of adipose tissue and the rest were divided into control and treatment groups. Procedures- The injury model was ...
متن کاملShift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction: Review of Upcoming Techniques
Standard isolation of adipose stromal vascular fraction (SVF) requires the use of collagenase and is considered more than "minimally manipulated" by current good manufacturing practice requirements. Alternatively, nonenzymatic isolation methods have surfaced using physical forces to separate cells from the adipose matrix. The purpose of this study was to review the literature on the use of mech...
متن کاملadipose derived stromal vascular fraction improves early tendon healing: an experimental study in rabbits
tendon never restores the complete biological and mechanical properties after healing. bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. stromal vascular fraction (svf), derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differ...
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ژورنال
عنوان ژورنال: Plastic and Reconstructive Surgery - Global Open
سال: 2020
ISSN: 2169-7574
DOI: 10.1097/gox.0000000000002652